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KMID : 0895820120220040037
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Journal of Oriental Rehabilitation Medicine
2012 Volume.22 No. 4 p.37 ~ p.57
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Effects of Kamchobuja-tang(Gancaofuzi-tang ) on Papain-Induced Osteoarthritis in Mice
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Choi Jae-Young
Oh Min-Seok
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Abstract
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Objectives : This study intends to clarify how Kamchobuja-tang(Gancaofuzi-tang, here in after referred to KBT) affect mice of C57BL/10 whose osteoarthritis was induced by papain.
Methods : Osteoarthritis was induced into mice by injecting papain in its knee joint. Mice are divided into a total of 4 groups(n=6). Normal group are not treated at all without inducing osteoarthritis whereas control group(OAC-control) were induced for osteoarthritis by papain and oral medicated with 200 ul of physiological saline per day. Positive comparison group(OAC-Joins®) was injected with papain and after 7 days, 100 mg/kg of Joins® was medicated with 200 ul of physiological saline mixed. Experimental group(OAC-KBT) was injected with papain and after 7 days that was medicated with 400 mg/kg of KBT mixed with 200 ul of physiological saline. OAC-Joins® and OAC-KBT were oral medicated for each substance a total of 4 weeks with one time per day. After experiments(from 1 week after injection of papain to 4 weeks elapsed), the functions of liver and kidney, inflammation cytokine values within serum, degree of revelation for inflammation cytokine genes, immune cells within blood, metabolism of arachidonic acid and amount of cartilage were measured and histopathological variations for knee joint structures were observed.
Results : 1. Functions of liver and kidney were not affected. 2. IL-6((interleukin-6), IL-1β(interleukin-1β), MCP-1(monocyte chemoattra- ctant protein-1) and TNF-α(tumor necrosis factor-α) are meaningfully reduced. 3. After analyzing inflammation cytokine in joints with mRMA(messenger ribonucleic acid), revelation of IL-1β, IL-6 and iNOS-Ⅱ(inducible nitric oxide synthase-Ⅱ) were all meaningfully reduced. And revelation of TNF-α and COX-2 gene were also reduced but meaningless. 4. Neutrophil and monocyte for WBC(white blood cell) within serum were meaningfully reduced. 5. PGE2(prostaglandin E2), TXB2(thromboxane B2) and LTB4(leukotriene B4) were meaningfully reduced. 6. Destruction of cartilage on micro CT(computed tomography)-arthrography was meaningfully reduced. 7. In terms of histopathology, infiltration of inflammation, proliferation of synovial membrane, subsidence of cartilage and bone due to penetration of excessive formation of synovial cell and destruction of cartilage were small(H&E(hematoxylin and eosin), Safranine-O staining).
Conclusions : Based on all results mentioned above, KBT is believed to be meaningful for suppressing the progress of osteoarthritis and its treatments because of its anti-inflammatory effects and alleviation of pain with histopathological effective efficacy.
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KEYWORD
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Kamchobuja-tang, Gancaofuzi-tang, Osteoarthritis, Papain-induced osteoarthritis, Cytokine, mRNA, Arachidonic acid
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